生物—电氧化法去除海水养殖循环水污染物

为提高海水养殖循环水处理效率,降低处理成本,本研究采用曝气生物滤器与电化学阳极氧化组合工艺,考察了不同阳极电势、进水氨氮和亚硝酸盐浓度下系统对氨氮及亚硝酸盐等污染物的去除效果,研究了微生物与工作电极之间的相互作用,并分析了电化学反应能耗。在水力停留时间为45 min、1.4 V阳极电压、进水氨氮和亚硝酸盐浓度分别为4.5和1.3 mg/L条件下,生物—电氧化法对氨氮去除率达88.8%,高出对照组7.6%,出水氨氮和亚硝酸盐浓度分别为0.5和0.9 mg/L,COD去除率为88.2%,高出对照组19.4%,平均能耗0.040 kWh/m~3,电极表面微生物生长对阳极电氧化过程有促进作用,微生物功能预测显示实验组硝化功能占比为0.03%,对照组为0.07%。研究表明,生物—电氧化法对海水养殖循环水的污染物有良好的去除效果,具有一定的发展应用潜力。     

过滤后养猪废水厌氧发酵与固氮技术研究

为探究养殖废水高效处理方法,文章采用粉碎玉米秸秆过滤养殖废水中悬浮性固体,并对过滤后养猪废水作厌氧发酵和固氮研究。经过滤,总固体(TS)去除率达47.55%,在中温(35±2)℃条件下,研究5、15、25 d 3种不同水力停留时间(HRT),过滤后养猪废水全混合厌氧发酵特性。厌氧发酵过程运行稳定,产生沼气甲烷含量均稳定在70%左右,当水力停留时间为15 d时,产甲烷效率为239.17 mL CH4/gVS,溶解性化学需氧量(SCOD)去除率60.94%。沼液采用鸟粪石沉淀法固氮处理,经过试验参数优化在物质的量比n(Mg2+)n(PO43-):n(NH4+)=1.021.061、pH为9.6条件下,氨氮(TAN)去除率达86.66%,处理后沼液中氨氮浓度为160 mg·L-1,研究结果可为养猪废水资源化利用提供参考。     

江西省水稻蓄雨间歇灌溉模式初探

【目的】探索水稻蓄雨间歇灌溉模式节水减排效益。【方法】以鄱阳湖区双季早晚稻为试验材料,采用大田和测坑试验,研究了水稻蓄雨间歇灌溉模式对灌溉定额、排水定额、降雨有效利用率、产量、稻田水分生产率,以及氮、磷排放量的影响,并与间歇灌溉和常规淹水灌溉试验进行了分析比较。【结果】与淹水灌溉、间歇灌溉相比,蓄雨间歇灌溉灌排水量、灌排次数明显减少。双季早晚稻年平均灌水量分别减少975m^3/hm^2和1251m^3/hm^2,年平均灌水次数分别减少8次和7.5次;年平均排水量分别减少729 m^3/hm^2和893 m^3/hm^2,年平均排水次数分别减少5.8次和3.1次;蓄雨间歇灌溉降雨有效利用率明显提高。早稻降雨利用率分别提高12.40%和9.14%,晚稻分别提高6.84%和6.42%;蓄雨间歇灌溉模式下,双季早晚稻总氮排放量年平均减排7.64 kg/hm^2和3.12 kg/hm^2,减排幅度34.93%和14.26%;双季早晚稻总磷排放量0.180kg/hm^2和0.095kg/hm^2,减排幅度37.25%和70.59%。【结论】蓄雨间歇灌溉模式具有明显的节水、减排和提高降雨有效利用率的效果,在我国南方多雨地区具有较强的推广应用空间。     

基于微流控和比色光谱法的水产养殖海水氨氮含量检测

为实时、准确地获取水产养殖海水中氨氮含量，以大围网养殖海水中的氨氮为研究对象，在比色光谱法基础上结合微流控技术进行靛酚蓝法比色反应，实现对溶液中氨氮含量的定量检测。建立数据处理后相应的模型，并且对比了不同光谱预处理和不同样本集划分算法对建立预测模型的影响，其中多元散色校正（MSC）后再使用小波平滑的预处理方法结合排序法划分样本集建立的偏最小二乘（PLS）回归模型效果最优，建模集校正标准差（RMSEC）和预测集校正标准差（RMSEP）分别为0.0566mg/L和0.0677mg/L，相对分析误差（RPD）为6.8932；在优化条件下测得方法的线性范围和检测限分别为0.005~1.350mg/L和0.0036mg/L。对海水、自来水和养殖水体进行加标回收实验，平均回收率在94%~109%之间，相对标准偏差在2.3%~5.8%之间。结果表明，实验建模效果良好，操作简单、方便，实验快速、可靠、无污染，表明利用比色光谱法结合微流控技术检测氨氮方法可行。     

Effects of three types of soil amendments on yield and soil nitrogen balance of maize-wheat rotation system in the Hetao Irrigation Area,China

Excessive fertilization combined with unreasonable irrigation in farmland of the Hetao Irrigation Area (HIR), China, has resulted in a large amount of nitrogen (N) losses and agricultural non-point source pollution. Application of soil amendments has become one of the important strategies for reducing N losses of farmland. However, there is still no systematic study on the effects of various soil amendments on N losses in the HIR. In this study, three types of soil amendments (biochar, bentonite and polyacrylamide) were applied in a maize-wheat rotation system in the HIR during 2015-2017. Yields of maize and wheat, soil NH₃ volatilization, N₂O emission and NO₃^- leaching were determined and soil N balance was estimated. The results showed that applications of biochar, bentonite and polyacrylamide significantly increased yields of maize by 9.2%, 14.3% and 13.3%, respectively, and wheat by 9.2%, 16.6% and 12.3%, respectively, compared with the control (fertilization alone). Applications of biochar, bentonite and polyacrylamide significantly reduced soil N leaching by 23.1%, 35.5% and 27.1%, soil NH₃-N volatilization by 34.8%, 52.7% and 37.8%, and soil N surplus by 23.9%, 37.4% and 30.6%, respectively. Applications of bentonite and polyacrylamide significantly reduced N₂O-N emissions from soil by 37.3% and 35.8%, respectively, compared with the control. Compared with application of biochar, applications of bentonite and polyacrylamide increased yields of maize and wheat by 5.1% and 3.5%, respectively. Our results suggest that soil amendments (bentonite and polyacrylamide) can play important roles in reducing N losses and increasing yield for the maize-wheat rotation system in the HIR, China.     

Effects of different tillage and straw retention practices on soil aggregates and carbon and nitrogen sequestration in soils of the northwestern China

Soil tillage and straw retention in dryland areas may affect the soil aggregates and the distribution of total organic carbon. The aims of this study were to establish how different tillage and straw retention practices affect the soil aggregates and soil organic carbon (SOC) and total nitrogen (TN) contents in the aggregate fractions based on a long-term (approximately 15 years) field experimentin the semi-arid western Loess Plateau, northwestern China. The experiment included four soil treatments, i.e., conventional tillage with straw removed (T), conventional tillage with straw incorporated (TS), no tillage with straw removed (NT) and no tillage with straw retention (NTS), which were arranged in a complete randomized block design. The wet-sieving method was used to separate four size fractions of aggregates, namely, large macroaggregates (LA, >2000 μm), small macroaggregates (SA, 250-2000 μm), microaggregates (MA, 53-250 μm), and silt and clay (SC, <53 μm). Compared to the conventional tillage practices (including T and TS treatments), the percentages of the macroaggregate fractions (LA and SA) under the conservation tillage practices (including NT and NTS treatments) were increased by 41.2%-56.6%, with the NTS treatment having the greatest effect. For soil layers of 0-5, 5-10 and 10-30 cm, values of the mean weight diameter (MWD) under the TS and NTS treatments were 10.68%, 13.83% and 17.65%, respectively. They were 18.45%, 19.15% and 14.12% higher than those under the T treatment, respectively. The maximum contents of the aggregate-associated SOC and TN were detected in the SA fraction, with the greatest effect being observed for the NTS treatment. The SOC and TN contents were significantly higher under the NTS and TS treatments than under the T treatment. Also, the increases in SOC and TN levels were much higher in the straw-retention plots than in the straw-removed plots. The macroaggregates (including LA and SA fractions) were the major pools for SOC and TN, regardless of tillage practices, storing 3.25-6.81 g C/kg soil and 0.34-0.62 g N/kg soil. Based on the above results, we recommend the NTS treatment as the best option to boost soil aggregates and to reinforce carbon and nitrogen sequestration in soils in the semi-arid western Loess Plateau of northwestern China.     

Nitrogen and phosphorus budget of a Haliotis discus hannai and Apostichopus japonicus polyculture system

The system nitrogen (N), phosphorous (P) budget, N/P utilization rate and the physiological response mechanism of the abalone Haliotis discus hannai (body weight: 12.87 ± 0.82 g) and the sea cucumber Apostichopus japonicus (body weight: 10.85 ± 1.16 g) to different co‐culture environment conditions were examined. Animals were kept in a multilayer, cubic recirculating aquaculture system at different polyculture densities (abalones at 400 ind/m²in monoculture [Group C] and abalones at 400 ind/m²with sea cucumbers at 10 ind/m² [AS1] or 20 ind/m² [AS2]). Each treatment was replicated four times, and the experimental cycle was 90 days. No significant difference in survival rate of abalones was detected when the stocking density of sea cucumbers increased from 10 to 20 ind/m², but the concentrations of total ammonia nitrogen and nitrite nitrogen in the water were significantly higher in AS2 than in AS1. Survival rate, specific growth rate (SGR) of body weight of sea cucumbers and SGR of body weight of abalones were significantly lower in AS2 than in AS1. No significant difference in protease (PES), lipase, amylase and cellulase activities of abalones was identified between Group C and AS1, but the PES and amylase activities of abalones and sea cucumbers in AS1 were significantly higher than those in AS2. In AS1, the N/P output from harvesting of abalones and sea cucumbers and the N/P utilization rates were significantly higher than those in AS2. Although the N/P output from faeces was significantly lower in AS2 than in Group C and AS1, the N/P output from the water layer was significantly higher than that in AS1. The expression levels of Cu/Zn superoxide dismutase (Cu/Zn‐SOD) and heat shock protein 70 (HSP70) of both abalones and sea cucumbers in AS2 were significantly higher than those in AS1. No significant difference in expression of catalase (CAT) and HSP90of abalones was identified among these groups, but the expression levels of CAT and HSP90of sea cucumbers in AS2 were significantly higher than those in AS1. These results indicate that stocking sea cucumbers at 10 ind/m² in the polyculture system will relieve the organic load on the system and improve the N/P utilization rate. It will also increase aquaculture production and improve the ecological and economic benefits of the system.     

Influence of intra-row cruciferous surrogate weed growth on crop yield in organic spring cereals

In Northern Europe, inter-row hoeing has become a popular tactic for controlling weeds in organic cereals. Hoeing is highly effective and can be implemented from crop emergence until stem elongation to maintain a nearly weed-free inter-row zone. However, hoeing has a lesser effect on weeds growing in the intra-row zone, where crop–weed proximity results in heightened competition. In the hoed cereal system, it is investigated whether tall-growing, competitive, cruciferous weeds in the intra-row zone affect crop biomass, yield and thousand kernel weight (TKW). An additive experimental design is employed to enable the fitting of rectangular hyperbolas, describing and quantifying the effects of increasing intra-row surrogate weed density on crop growth parameters. Regressions were studied under the influence of crop (spring barley and spring wheat), row spacing (narrow [12.5 or 15.0 cm] and wide [25.0 cm]) and nitrogen rate (50 and 100 kg NH₄-N/ha). Cruciferous surrogate weeds were found to impact crop yield and quality severely. For example, ten intra-row plants/m² of surrogate weed Sinapis alba reduced grains yields by 7%–14% in spring barley and by 7%–32% in spring wheat with yield losses becoming markedly greater in wheat compared to barley as weed density increases. Compared to wheat, barley limited yield and quality losses and suppressed intra-row weed growth more. Row spacing did not have a consistent effect on crop or weed parameters; in one of six experiments, the 25 cm row spacing reduced yields and increased intra-row weed biomass in wheat. Nitrogen rate did not affect crop or weed parameters. Results warrant the implementation of additional tactics to control intra-row weeds and limit crop losses.     

灌浆温度和氮肥及其互作效应对稻米贮藏蛋白组分的影响

灌浆结实期温度与氮肥施用量是影响稻米品质的两个重要生态因子,尤其是与稻米蛋白含量及米饭食味关系密切。本文以多个水稻主栽品种为材料,通迆灌浆结实期的人工控温试验、大田长期定位点的施氮处理试验和盆栽条件下的温氮两因素复合处理试验,探讨了水稻灌浆结实期温度对稻米贮藏蛋白含量与组分影响及其有别于氮肥处理效应的差异觃律,幵分析了温度与氮肥两个因素对稻米贮藏蛋白及其组分影响的交互作用特点。结果表明,高温胁迫和增施氮肥均引起水稻籽粒总蛋白及其谷蛋白组分含量(%)的显著增加,但两者对稻米醇溶蛋白影响却存在明显差别。其中,高温处理引起醇溶蛋白含量显著下降,提高稻米谷蛋白/醇溶蛋白比值,而增施氮肥引起稻米谷蛋白和醇溶蛋白含量明显增加,但对谷蛋白/醇溶蛋白比值与贮藏蛋白各亚基的组成比例影响相对较小。在高温处理下,谷蛋白的57kD前体亚基组分含量有所提高,而37kD酸性亚基和22kD碱性亚基随温度处理的差异变化却因品种而异,且高温处理对水稻籽粒蛋白绝对含量(mg grain~(–1))的影响程度也进没有其对蛋白相对含量(%)的影响明显。高氮×高温处理组合对稻米总蛋白与谷蛋白含量的影响程度显著大于单一高温或高氮处理,但在高氮水平下由高温引起稻米醇溶蛋白含量的下降幅度却小于其低氮对照,有利于稻米醇溶蛋白含量在不同温度处理下的相对稳定。     

基于评价模型的宁夏沙土春玉米最佳灌水施氮量研究

为分析不同水氮供应对宁夏沙土春玉米主要评价指标的影响,采用灌水和施氮2因素交互设计,灌水量设置3个水平(W0.6(0.6KcET0)、W0.8(0.8KcET0)和W1.0(KcET0),Kc为作物系数,ET0为潜在作物蒸发蒸腾量),施氮量设量4个水平:N150(150 kg/hm2)、N225(225 kg/hm2)、N300(300 kg/hm2)和N375(375 kg/hm2),进行了大田试验。结果表明:灌水量和施氮量的交互作用对产量、水分利用效率(WUE)和氮肥偏生产力(PFPN)有极显著影响,对地上部干物质累积量和籽粒氮素累积量有显著影响;在相同灌水条件下,春玉米地上部干物质累积量、产量、WUE均随施氮量的增加先增加后减小。主成分分析法、隶属函数分析法、灰色关联度分析法与基于组合赋权的TOPSIS模型两两之间具有良好的相关性,各模型之间相关系数均值为0.465～0.787;基于整体差异组合评价模型得出W0.8N300评价值最高。考虑试验区年际降雨量分布不均,经回归分析拟合得出,当春玉米生育期内灌水量与有效降雨量之和为544 mm、施氮量为260 kg/hm2时,春玉米综合指标评价值最高(1.47),为适宜的春玉米滴灌灌水施肥量。本研究可为沙土地区春玉米滴灌施肥过程中水氮科学管理提供指导依据。